The Prolonged Life-Span of Alveolar Macrophages

• DOI: 10.1165/rcmb.2007-0224rc
• Publication Date: 2008-01-11T02:18:58Z
• Authors: Jaime Murphy; Ross Summer; Andrew A. Wilson; Darrell N. Kotton; Alan Fine
• Keywords: Alveolar macrophage

To further examine the half-life of alveolar macrophages, chimeric CD 45.2 mice were generated through bone marrow transplantation donor 45.1 cells. Before administration cells, recipient divided into two cohorts: first cohort received total body irradiation; second also irradiation-however, thorax, head, and upper extremities shielded with lead. Flow cytometric analysis was then performed on blood, peritoneal, bronchoalveolar lavage cells over time to quantify engraftment. The data for unshielded revealed a macrophage 30 days. In cohort, however, we found that by 8 months there negligible replacement macrophages despite reconstitution blood peritoneum marrow. Consistent these findings, mean fluorescent intensity remained stable 4-week period after in vivo PKH26 dye loading. Together, show previous studies confounded fact they did not account toxic effects irradiation conditioning regimens, demonstrate does significantly contribute compartment during steady-state conditions.