Preparation and Characterization of RGDS/Nanodiamond as a Vector for VEGF-siRNA Delivery

• DOI: 10.1166/jbn.2015.2005
• Publication Date: 2014-08-22T21:18:53Z
• Authors: Chunying Cui; Yuji Wang; Kaikai Yang; Yaonan Wang; Junyu Yang; Jianzhong Xi; Ming Zhao; Jianhui Wu; Shiqi Peng
• Keywords: Nanodiamond

Small interfering RNA (siRNA) has great potential for treating or preventing diseases. Safe and efficient vectors are extremely needed specific delivery of siRNA. Here VEGF-siRNA/RGDS/nanodiamond was prepared by conjugating Arg-Gly-Asp-Ser (RGDS) VEGF-siRNA to nanodiamond particles. could be clinically used in anti-angiogenic gene therapy inhibit tumor growth via the down-regulation expression vascular endothelial factor (VEGF). The differential scanning calorimeter (DSC) analysis evidenced that RGDS and/or were effectively linked nanodiamond. release assays indicated presence time prolonged 6 folds. This enabled transfer a long-acting manner, thereby significantly decrease VEGF mRNA protein. Real-Time PCR revealed decreased 87.56 ± 1.6% VEGF-siRNA/RGDS/nanodiamond. Enzyme-Linked Immunosorbent Assay (ELISA) protein down-regulated 39.8 1.8%. also observed Western blotting experiments. In human umbilical vein cells (HUVEC) test, formation tubes exhibited no testable cytotoxicty. All results consistently suggested RGDS/nanodiamond is an ideal non-viral tumor-targeting vector siRNA transfer, may promising regimen carcinoma.