Significant Toxic Effect of Carbon Nanofibers at the Early Stage of Embryogenesis

• DOI: 10.1166/jbn.2020.2937
• Publication Date: 2020-11-14T04:49:36Z
• Authors: Ghada G. Abdo; Hadeel Kheraldine; Ishita Gupta; Balsam Rizeq; Ahmed Elzatahry; Ala-Eddin Al Mous...; Ashraf Khalil
• Keywords: Chorioallantoic membrane

Implementation of carbon nanofibers (CNFs) in biomedical applications have successful outcomes, however, they are still considered as a potential hazard. We herein used avian embryos at 3 days and its chorioallantoic membrane (CAM) 6 incubation to evaluate the impact synthesized CNFs on early stage embryogenesis angiogenesis. Our data point out that 50 μg/embryo concentration provoke adverse effects 75% CNFs-exposed die within 1-5 after exposure compared with their matched controls. Furthermore, significantly inhibit angiogenesis CAM 48-hours post-treatment. Additionally, RT-PCR analysis seven key controller genes responsible for proliferation, survival, angiogenesis, apoptosis showed these deregulated brain, heart, liver tissues control. investigation suggests could toxic effect stages well Nevertheless, further investigations required elucidate mechanism normal development human health.