Circ-AKT3 Inhibits Malignant Progression of Hepatocellular Carcinoma by Negatively Regulating PDK1

• DOI: 10.1166/jbn.2024.3751
• Publication Date: 2024-01-19T04:35:31Z
• Authors: Feng Dong; Chunlin Ke; Peirong Wang; Biao Chen; Chuanshu Cai
• Keywords: AKT3; Tumor progression

This study investigated the clinical and functional significance of circ-AKT3 in hepatocellular carcinoma (HCC) its impact on tumor progression. Using qRT-PCR, we found reduced levels HCC tissues compared to adjacent liver samples. Clinical data revealed that low expression correlated with increased lymphatic distant metastasis. Functional experiments MHCC-97H Hep3B cells, chosen due their levels, demonstrated overexpression suppressed cell proliferation, invasion, migration. Bioinformatics analysis luciferase assays indicated potentially targets PDK1. PDK1 was elevated inversely circ-AKT3, upon overexpression. Rescue illustrated reversed circ-AKT3-induced inhibition proliferation In conclusion, downregulated HCC, associated aggressive features, hinders progression by modulating suggests circ-AKT3′s potential as a diagnostic prognostic biomarker for HCC.