Adipose Mesenchymal Stem Cell-Derived Exosomes Promote the Regeneration of Corneal Endothelium Through Ameliorating Senescence

• DOI: 10.1167/iovs.64.13.29
• Publication Date: 2023-10-18T14:31:18Z
• Authors: Yunkyoung Ryu; Jin Sun Hwang; Kyung Bo Noh; Se Hie Park; Je Hyun Seo; Young Joo Shin
• Keywords: Adherens junction; Exosome

Human corneal endothelial cells (hCECs) have been considered unable to regenerate in vivo, resulting decompensation after significant loss of hCECs. adipose-derived mesenchymal stem cell (ASC)-derived exosomes can tissues and organs. In this study, we investigated whether ASC-derived could protect CECs.We performed viability cell-cycle analyses evaluate the effect on regeneration capacity cultured Transforming growth factor-β (TGF-β) hydrogen peroxide (H2O2) were used induce biological stress CECs. The CECs was vivo. introduced into rat using electroporation, corneas injured cryoinjury. Next-generation sequencing analysis compare differentially expressed microRNAs (miRNAs) between hCEC-derived exosomes.ASC-derived induced CEC proliferation suppressed TGF-β- or H2O2-induced oxidative senescence. hCECs against endothelial-mesenchymal transition mitophagy. an vivo promoted wound healing protected endothelium cryoinjury-induced damage. revealed miRNAs for exosomes. They are involved lysine degradation, adherens junction, TGF-β signaling pathway, p53 Hippo forkhead box O (FoxO) regulation actin cytoskeleton, RNA degradation based Kyoto Encyclopedia Genes Genomes (KEGG) pathway analysis.ASC-derived by inducing a shift cycle suppressing senescence autophagy.