In Vivo Imaging of Newt Lens Regeneration: Novel Insights Into the Regeneration Process

• DOI: 10.1167/tvst.10.10.4
• Publication Date: 2021-08-12T14:52:54Z
• Authors: Weihao Chen; Georgios Tsissios; Anthony Sallese; Byran Smucker; Anh-Thu Nguyen; Junfan Chen; Hui Wang; Katia Del Rio-Tsonis
• Keywords: Planarian

Purpose: To establish optical coherence tomography (OCT) as an in vivo imaging modality for investigating the process of newt lens regeneration. Methods: Spectral-domain OCT was employed regeneration process. A total 37 newts were lentectomized and followed by over course 60 to 80 days. Histological images obtained at several time points compare with corresponding images. Volume measurements also acquired. Results: can identify key features observed histological based on scattering differences from various eye tissues, such cornea, intact regenerated lens, iris. Lens volume three-dimensional showed that regenerating size increased linearly until days post-lentectomy. Conclusions: Using imaging, we able track entire first time. Three-dimensional allowed us volumetrically quantify visualize dynamic spatial relationships between tissues during Our results track/analyze same animal. Translational Relevance: represents a unique example vertebrate tissue plasticity. Investigating cellular morphological events govern this extraordinary will advance our understanding shed light developing new therapies treat blinding disorders higher vertebrates.