Imaging Melanin Distribution in the Zebrafish Retina Using Photothermal Optical Coherence Tomography

• DOI: 10.1167/tvst.7.5.4
• Publication Date: 2018-09-07T15:42:12Z
• Authors: Maryse Lapierre-L...; Alison L. Huckenp...; Brian A. Link; Ross F. Collery; Joseph Carroll; Melissa C. Skala
• Keywords: Melanosome

To demonstrate and validate that photothermal optical coherence tomography (PT-OCT) can image melanin in the retinal pigment epithelium (RPE) observe light-driven melanosome translocation zebrafish retina.A commercial spectral domain OCT system was modified to perform both PT-OCT. Four adult tyrosinase-mosaic with varying levels of expression across their retinas were imaged, PT-OCT signal for pigmented nonpigmented regions compared. Wild-type dark-adapted (n = 11 fish) light-adapted 10 also imaged Longitudinal reflectivity absorption profiles generated from B-scans compare distribution between two groups.A significant increase (P < 0.0001, Student's t-test) observed interest (ROI) compared ROIs zebrafish, which confirms is specific eye. A intensity detected wild-type group group. Additionally, display more distinct banding patterns than do B-scans.PT-OCT detect different characterize retina, including intracellular changes due within RPE.PT-OCT could quantify pigmentation occur diseases. The functional information provided by may enable a better understanding anatomical features conventional images.