Absence of Desmin in Myofibers of the Zebrafish Extraocular Muscles

• DOI: 10.1167/tvst.9.10.1
• Publication Date: 2020-09-01T18:26:24Z
• Authors: Nils Dennhag; Jing-Xia Liu; Hanna Nord; Jonas von Hofsten; Fatima Pedrosa Do...
• Keywords: Desmin; Immunolabeling; Danio

Purpose: To study the medial rectus (MR) muscle of zebrafish (Danio rerio) with respect to pattern distribution desmin and its correlation distinct types myofibers motor endplates. Methods: The MRs were examined using confocal microscopy in whole-mount longitudinal specimens cross sections processed for immunohistochemistry antibodies against desmin, myosin heavy chain isoforms, innervation markers. Desmin patterns correlated major myofiber type innervation. A total 1382 nine MR muscles analyzed. Results: Four immunolabeling found MRs. Approximately a third all slow lacked representing 8.5% population. adult displayed en grappe, plaque, multiterminal plaque neuromuscular junctions (NMJs) intricate immunolabeling. Conclusions: showed important similarities human extraocular regard presence NMJs can be regarded as an adequate model further role implications heterogeneity cytoskeletal protein composition. Translational Relevance: establishment cytoskeleton that are particularly resistant disease opens new avenues understand myopathies dystrophies may provide clues therapies.