Longitudinal adaptive optics fluorescence microscopy reveals cellular mosaicism in patients

• DOI: 10.1172/jci.insight.124904
• Publication Date: 2019-03-23T13:59:51Z
• Authors: HaeWon Jung; Jianfei Liu; Tao Liu; Aman George; Margery G. Smelki...; Sarah Cohen; Ruchi Sharma; Owen Schwartz; Arvydas Maminishkis; Kapil Bharti; Catherine Cukras; Laryssa A. Huryn; Brian P. Brooks; Robert Fariss; Johnny Tam
• Keywords: Indocyanine Green; Ex vivo; Fluorescence-lifetime imaging microscopy

The heterogeneity of individual cells in a tissue has been well characterized, largely using ex vivo approaches that do not permit longitudinal assessments the same over long periods time. We demonstrate potentially novel application adaptive optics fluorescence microscopy to visualize and track situ mosaicism retinal pigment epithelial (RPE) directly human eye. After short, dynamic period during which RPE take up i.v.-administered indocyanine green (ICG) dye, we observed remarkably stable fluorescent pattern gradually disappeared days. This could be robustly reproduced with new injection follow-up imaging eye out at least 12 months, enabled tracking cells. Investigation ICG uptake primary mouse model alongside corroborated our findings is an intrinsic property tissue. detect subclinical changes RPE, technical advance direct implications for improving understanding diseases such as oculocutaneous albinism, late-onset degeneration, Bietti crystalline dystrophy.