Mass cytometry identifies distinct CD4+ T cell clusters distinguishing HIV-1–infected patients according to antiretroviral therapy initiation

• DOI: 10.1172/jci.insight.125442
• Publication Date: 2019-02-06T16:01:05Z
• Authors: Yonas Bekele; Tadepally Lakshmi...; Yang Chen; Jaromir Mikes; Aikaterini Nasi; Stefan Petkov; Bo Hejdeman; Petter Brodin; Francesca Chiodi
• Keywords: Mass cytometry

Recent guidelines recommend antiretroviral therapy (ART) to be administered as early possible during HIV-1 infection. Few studies addressed the immunological benefit of commencing ART acute phase We used mass cytometry characterize blood CD4+ T cells from HIV-1–infected patients who initiated or chronic Using this method, we analyzed a large number markers on millions individual immune cells. The results revealed that cell clusters with high expression CD27, CD28, CD127, and CD44, whose function involves migration inflamed tissues survival, are more abundant in healthy controls initiating phase; contrary, had reduced these markers. suggestive better preserved