The distinct metabolism between large and small HDL indicates unique origins of human apolipoprotein A4

• DOI: 10.1172/jci.insight.162481
• Publication Date: 2023-04-23T18:01:05Z
• Authors: Allison B. Andraski; Sasha A. Singh; Hideyuki Higashi; Lang Ho Lee; Masanori Aikawa; Frank M. Sacks
• Keywords: Chylomicron

Apolipoprotein A4's (APOA4's) functions on HDL in humans are not well understood. A unique feature of APOA4 is that it an intestinal apolipoprotein secreted and chylomicrons. The goal this study was to gain a better understanding the origin function by studying its metabolism across 6 sizes. Twelve participants completed metabolic tracer study. isolated APOA1 immunopurification separated size. Tracer enrichments for were determined targeted mass spectrometry, rates derived compartmental modeling. small (alpha3, prebeta, very prebeta) distinct from large (alpha0, 1, 2). appeared circulation 30 minutes relatively rapidly catabolized. In contrast, later (1–2 hours) had much slower catabolism. profiles likely indicate each has humans. This evidence supports notion originates directly intestine while chylomicron transfer.