Insulin mitigates acute ischemia–induced atrial fibrillation and sinoatrial node dysfunction ex vivo

• DOI: 10.1172/jci.insight.185961
• Publication Date: 2024-11-14T17:00:19Z
• Authors: Huiliang Qiu; Fan Li; Hannah Prachyl; Alejandra Patino-...; Michael Rubart; Wuqiang Zhu
• Keywords: Ex vivo

Acute atrial ischemia is a well-known cause of postoperative fibrillation (POAF). However, mechanisms through which contributes to the development POAF are not well understood. In this study, ex vivo Langendorff perfusion was used induce acute and reperfusion in heart order mimic POAF. Inducibility (AF) evaluated using programmed electrical stimulation confirmed with open-atrium optical mapping. Compared control group without ischemia, 25 minutes substantially increased incidence AF. The right atrium more susceptible AF than left atrium. Administering insulin for 30 before during greatly reduced vulnerability treatment only did show substantial benefits against Optical mapping studies showed that mitigates ischemia-induced abnormal electrophysiology, including shortened action potential duration effective refractory period, slowed conduction velocity, heterogeneity, altered calcium transients. conclusion, risk ischemia/reperfusion-induced via improving electrophysiology handling cardiomyocytes, provides therapy