Bioactive Nanofibrous Scaffolds for Regenerative Endodontics

• DOI: 10.1177/0022034513505770
• Publication Date: 2013-09-21T06:27:02Z
• Authors: M.C. Bottino; K. Kamocki; G.H. Yassen; J.A. Platt; M.M. Vail; Y. Ehrlich; K.J. Spolnik; R.L. Gregory
• Keywords: Enterococcus faecalis

Here we report the synthesis, materials characterization, antimicrobial capacity, and cytocompatibility of novel antibiotic-containing scaffolds. Metronidazole (MET) or Ciprofloxacin/(CIP) was mixed with a polydioxanone (PDS)polymer solution at 5 25 wt% processed into fibers. PDS fibers served as control. Scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), tensile testing, high-performance liquid chromatography (HPLC) were used to assess fiber morphology, chemical structure, mechanical properties, drug release, respectively. Antimicrobial properties evaluated against those Porphyromonas gingivalis/Pg Enterococcus faecalis/Ef. Cytotoxicity assessed in human dental pulp stem cells (hDPSCs). Statistics performed, significance set 5% level. SEM imaging revealed submicron diameter. FTIR confirmed antibiotic incorporation. The values hydrated CIP scaffold significantly lower than all other groups. Analysis HPLC data gradual, sustained release from scaffolds over 48 hrs. CIP-containing ( p < .00001) inhibited biofilm growth both bacteria. Conversely, MET-containing only Pg growth. Agar diffusion specific bacteria for Only cytotoxic. Collectively, this study suggests that polymer-based electrospun could function biologically safe delivery system regenerative endodontics.