Peripheral Blood and Tumor Infiltrating Lymphocytes in Non-Small Cell Lung Cancer: Analysis at the Population and Clonal Level

• DOI: 10.1177/030089169007600515
• Publication Date: 2018-04-17T09:36:26Z
• Authors: Maurizio Viale; Silvano Ferrini; Salvatore Serrano; Davide Serrano; Andrea Ardizzoni; Angelo Nicolin
• Keywords: Tumor-infiltrating lymphocytes

Tumor infiltrating (TIL) and peripheral blood lymphocytes (PBL) were isolated from 18 patients with non-small cell lung cancer undergoing radical surgery. Surface marker analysis revealed that TILs PBLs mainly consisted of CD3+ T cells generally displayed a lower CD4/CD8 ratio. Differences found in the expression CD25 (IL-2 receptor) DR (MHC class II) antigens, which increased TILs, percentage CD16+ natural killer (NK) cells, was reduced as compared to PBLs. Accordingly, NK activity than PBLs, whereas neither nor expressed spontaneous cytolytic against fresh autologous tumor melanoma "NK-resistant" A549 carcinoma line. After 4 days culture medium recombinant-interleukin-2 (rIL-2), acquired all targets, but higher levels cytotoxicity only 3 out 16 tested. More importantly, both similar cytotoxic cells. 8 also analyzed by limiting dilution microculture system. Cloning efficiency remarkably surface clones confirmed an accumulation CD8+ lymphocytes, lectin-dependent assay, occurred at site. The non-MHC-restricted TIL- PBL-derived K562, A549, allogeneic showed no significant differences. Only 53% TIL released IL-2 response PHA + TPA stimulation, 68% producers. Moreover, most PBL- TIL-derived necrosis factor alpha mitogen stimulation.