Differentiation of mesenchymal stem cells into neuron-like cells using composite 3D scaffold combined with valproic acid induction

• DOI: 10.1177/0885328217741903
• Publication Date: 2017-11-24T07:17:23Z
• Authors: Sadegh Ghorbani; Taki Tiraihi; Masoud Soleimani
• Keywords: Wharton's jelly; Neural tissue engineering

The nervous system has little capacity for self-repair after injury because neurons cannot proliferate owing to lack of suitable microenvironment. Therefore, neural tissue engineering that combines stem, scaffolds, and growth factors may improve the chance restoration damaged tissues. A favorable niche regeneration would be both fibrous electrically conductive scaffolds. Human Wharton jelly-derived mesenchymal stem cells were seeded on wet-electrospun 3D scaffolds composed poly lactic acid coated with natural polymers including alginate gelatin, followed by a multi-wall carbon nanotube coating. results show scaffold at concentration 15% w/v had higher porosity (above 80%) than other concentrations. Moreover, supported human in culture, incubated 21 days 1 mM valproic as inducer resulted improvement differentiation into neuron-like immunoreactivity nestin, Map2, neuron specific enolase (NSE), which also consistent reverse transcription polymerase chain reaction (RT-PCR) quantitive Reverse (qRT-PCR) results. conclusion is composite nanofiber improved transdifferentiation cells.