A novel natural-derived tilapia skin collagen mineralized with hydroxyapatite as a potential bone-grafting scaffold

• DOI: 10.1177/08853282221086246
• Publication Date: 2022-03-29T08:30:22Z
• Authors: Shiyu Yao; Yuli Shang; Bo Ren; Shu Deng; Zhe Wang; Yang Peng; Zhaohui Huang; Shiqing Ma; Cheng Peng; Shuai Hou
• Keywords: Biocompatibility; Carbodiimide; Biomaterial; Mechanical strength

Collagen is widely used in medical field because of its excellent biocompatibility and bioactivity. To date, collagen for biomedical use always derived from bovine or swine. The purpose this study was to evaluate collagen-based biomaterials non-mammalian donors bone repair. Thus, tilapia skin collagen-hydroxyapatite (T-col/HAp) scaffolds were fabricated three different proportions then cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-N-hydroxysuccinimide (EDC-NHS). evaluated their microstructure, chemical physical properties, mechanical strength degradability. Then the vitro responses mesenchymal stem cells (BMSCs) investigated terms cellular proliferation, differentiation, mineralization. At last, implanted into rat skull critical defections investigate potential osteogenic activities. As a result, pore sizes porosities approximately 106.67-196.67 μm 81.5%-66.7%. Pure group showed 0.065 MPa, significantly enhanced almost 17 times 32 collagen/HAp ratio 1:4 1:9 groups. In studies revealed most prominent healthy growth BMSCs group. All certain activities those loaded small amount hydroxyapatite strongest bioactivities. micro-CT that defect filled generated 6 months after implantation biomechanics tests further confirmed highest This indicated aquatic might be alternative type I mammals tissue engineering. combination inorganic materials also important appropriate component loading can achieve both quality efficiency extent.