Myelin water imaging in multiple sclerosis: quantitative correlations with histopathology

• DOI: 10.1177/1352458506070928
• Publication Date: 2006-12-05T12:14:10Z
• Authors: C Laule; E Leung; D KB Li; A L Traboulsee; D W Paty; A L MacKay; G RW Moore
• Keywords: Histopathology; Remyelination

Various magnetic resonance (MR) techniques are used to study the pathological evolution of demyelinating diseases, such as multiple sclerosis (MS). However, few studies have validated MR derived measurements with histopathology. Here, we determine correlation myelin water imaging, an measure content, quantitative histopathologic measures density. The multi-component T 2 distribution was determined from 25 formalin-fixed MS brain samples using a multi-echo relaxation experiment. fraction (MWF), defined signal below 30 milliseconds divided by total signal, for various regions interest and compared Luxol fast blue (myelin stain) mean optical density (OD) each sample. MWF had strong stain [mean (range) R -/0.67 (0.45+ 0.92)], validating This technique has many practical applications in vivo monitoring demyelination remyelination variety disorders myelin.