Detection and Identification of Potential Biomarkers of Non-small Cell Lung Cancer

• DOI: 10.1177/153303460900800607
• Publication Date: 2014-10-03T18:55:00Z
• Authors: Yang Yang; Song Zhao; Yuxia Fan; Feng Zhao; Quan Liu; Wei Hu; Donglei Liu; Kai Fan; Jiaxiang Wang; Jianjun Wang
• Keywords: Biomarker Discovery; Haptoglobin

The aim of this study was to discover and identify potential protein biomarkers specific for non-small cell lung cancer (NSCLC). Two hundred thirty five (235) Serum samples with 112 NSCLC 123 controls were randomly divided into a training set blind testing set. proteomic profiles analyzed using surface-enhanced laser desorption/ionization time-of-flight mass spectroscopy (SELDI-TOF-MS). Candidate purified by high Performance Liquid Chromatography (HPLC) identified liquid chromatography tandem spectrometry (LC-MS/MS) validated ProteinChip immunoassays. A total 3 peaks (m/z 6628, 9191 11412 Da) screened out SVM construct the classification model discriminatory power in sensitivity specificity 96.56% 94.79% respectively candidate biomarker m/z 6628 Da found down-regulated patients, as apolipoprotein C-I. Another two (9191 up-regulated serum patients haptoglobin alpha-1 chain S100A4, respectively. We have that could discriminate from non-cancer controls. An efficient strategy, including SELDI-TOF-MS analysis, HPLC purification, MALDI-TOF-MS trace LC-MS/MS identification, has been proved successfully.