The first 3 days of B-cell development in the mouse embryo

• DOI: 10.1182/blood-2002-03-0809
• Publication Date: 2002-11-14T17:42:59Z
• Authors: Belén de Andrés; Pilar Gonzalo; Susana Minguet; José A. Martı́ne...; Pilar G. Soro; Miguel Angel R. M...; Marı́a Luisa Gaspar
• Keywords: 0301 basic medicine; B-Lymphocytes; Mice, Inbred BALB C; Time Factors; Lymphopoiesis; Antigens, CD19; Cell Differentiation; Hematopoietic Stem Cells; Mice; 03 medical and health sciences; Liver; Mesonephros; Animals; Cell Lineage; Gonads; Aorta

B-lineage–committed cells are believed to arise in the liver of mouse embryos at 14 days after coitus (dpc). However, pre-B-specific gene transcripts and DJH gene rearrangements have been detected in earlier, midgestation embryos. We describe here a population of c-kit+AA4.1+CD19+Pax5+cells present in the aorta-gonad-mesonephros (AGM) area and in the livers of 11-dpc mouse embryos. In contrast to multipotent c-kit+AA4.1+CD19−hematopoietic stem cells (HSCs), these c-kit+AA4.1+CD19+ progenitors differentiated only to B-lineage cells in vitro. We propose that mouse embryonic B lymphopoiesis starts earlier than previously thought, at 10 to 11 dpc, both in liver and extra-liver hematopoietic sites. The B-cell differentiation program is not delayed with respect to the emerging lymphohematopoiesis events in the midgestation mouse embryo (8-9 dpc).