Proliferation of early human myeloid precursors induced by interleukin- 1 and recombinant soluble CD23

• DOI: 10.1182/blood.v75.10.1924.1924
• Publication Date: 2019-10-13T20:52:17Z
• Authors: MD Mossalayi; M Arock; JM Bertho; C Blanc; AH Dalloul; H Hofstetter; M Sarfati; G Delespesse; P Debre
• Keywords: CD23; Interleukin 3

Abstract Low affinity Fc epsilon receptors (Fc epsilon RII/CD23) or their soluble fragments have various biologic effects on B- and T-cell lineages. In this study, we have assessed the effect of recombinant soluble CD23 (rsCD23) on the proliferation of human bone marrow (BM)- derived myeloid precursors with or without recombinant interleukin-1 (rIL-1) addition. Non-adherent CD2- or CD34+ BM cell subsets were used as target cells. Our results show that rsCD23 in synergy with rIL-1 displays an interleukin-3-like activity as it promotes the proliferation of multipotential marrow precursors. This effect was abolished by anti-CD23 addition to these cultures, but was not affected by anti-IL-3 monoclonal antibody. Furthermore, sequential study indicates that rIL-1 induces bone marrow cell responsiveness to rsCD23.