The role of short-chain dehydrogenase/oxidoreductase, induced by salt stress, on host interaction of B. pseudomallei

• DOI: 10.1186/1471-2180-14-1
• Publication Date: 2014-01-02T10:01:14Z
• Authors: Pornpan Pumirat; Usa Boonyuen; Muthita Vanaporn; Peechanika Pinweha; Sarunporn Tandhav...; Sunee Korbsrisate; Narisara Chantratita
• Keywords: Burkholderia pseudomallei; Melioidosis

Burkholderia pseudomallei is the causative agent of melioidosis, a frequently occurring disease in northeastern Thailand, where soil and water high salt content are common. Using microarray analysis, we previously showed that B. up-regulated short-chain dehydrogenase/oxidoreductase (SDO) under stress. However, importance SDO infection unknown. This study aimed to explore function SDO, investigate its role interactions between host cells. Bioinformatics analysis structure, based on homology modeling, revealed NAD+ cofactor domain catalytic triad containing Ser149, Tyr162, Lys166. similar Bacillus megaterium glucose 1-dehydrogenase. To this protein, constructed defective mutant, measured dehydrogenase (GDH) activity, tested with The K96243 wild type exhibited potent GDH activity condition 300 mM NaCl, while mutant levels 15 times lower. Both invasion into A549 cell line early intracellular survival within J774A.1 macrophage were impaired mutant. Complementation was able restore ability produce invade epithelial cells, survive macrophages. Our data suggest induced during stress may facilitate affect initiation successful infection. Identifying provides better understanding association bacterial adaptation pathogenesis melioidosis.