B cell Semaphorin 4c expression mitigates the airway hyperresponsiveness and acute inflammation which characterize allergic airway disease

• DOI: 10.1186/1710-1492-10-s2-a26
• Publication Date: 2015-06-18T11:17:35Z
• Authors: Alex Lei; Di Xue; Marylin Desjardins; Marianne Beland; Bruce Mazer
• Keywords: Pulmonary and Respiratory Medicine; 0301 basic medicine; 03 medical and health sciences; Meeting Abstract; Immunology and Allergy; 3. Good health

Methods Wild-type and Sema4C mice were sensitized intraperitoneally using 100 μL OVA (0.5 mg/mL ovalbumin and 4 mg/mL aluminum hydroxide in PBS) on days 0 and 14, and were challenged intranasally using 20 μL OVA (10 mg/mL ovalbumin in PBS) from days 28 to 30. Sacrifice and analysis of Airway Hyperresponsiveness via flexiVent was performed on day 31. Serum IgE and IL-10 expression levels were measured by ELISA. B cells were phenotyped by fluorescence-activated cell sorting (FACS). B cell motility was measured by migration assays.