Increased plasma disequilibrium between pro- and anti-oxidants during the early phase resuscitation after cardiac arrest is associated with increased levels of oxidative stress end-products

• DOI: 10.1186/s10020-021-00397-x
• Publication Date: 2021-10-24T19:02:37Z
• Authors: Muhammad Shoaib; Nancy Kim; Rishabh C. Choudhary; Tai Yin; Koichiro Shinozaki; Lance B. Becker; Junhwan Kim
• Keywords: Malondialdehyde

Abstract Background Cardiac arrest (CA) results in loss of blood circulation to all tissues leading oxygen and metabolite dysfunction. Return flow during resuscitative efforts is the beginning reperfusion injury marked by generation reactive species (ROS) that can directly damage tissues. The plasma serves as a reservoir transportation medium for metabolites critical survival well ROS are generated. However, complicated interplay among various antioxidant counterparts, particularly after CA, have not been evaluated. In this study, we assessed equilibrium between pro- anti-oxidants within assess oxidative status post-CA. Methods male Sprague–Dawley rats, 10 min asphyxial-CA was induced followed cardiopulmonary resuscitation (CPR). Plasma drawn immediately achieving return spontaneous (ROSC) 2 h post-ROSC. isolated analyzed prooxidant capacity (Amplex Red dihydroethidium oxidation, total nitrate nitrite concentration, xanthine oxidase activity, iron concentration) (catalase superoxide dismutase activities, Total Antioxidant Capacity, Iron Reducing Power Assay). consequent products, such 4-Hydroxyl-2-noneal, malondialdehyde, protein carbonyl, nitrotyrosine were evaluated determine degree damage. Results After CA resuscitation, two trends observed: (1) lower ischemia, but rapidly increased post-ROSC compared control, (2) either decreased or did increase substantially control. Consequently, oxidation products Conclusion Our study disbalance early phase resuscitation. This disequilibrium favors prooxidants associated with levels downstream stress-induced end-products, which body’s unable mitigate. Here, suggest circulating major contributor stress post-CA its management requires substantial intervention favorable outcomes.