Lnc-DC regulates cellular turnover and the HBV-induced immune response by TLR9/STAT3 signaling in dendritic cells

• DOI: 10.1186/s11658-018-0108-y
• Publication Date: 2018-09-03T12:25:06Z
• Authors: Lifan Zhuang; Jianhua Tian; Xinzhi Zhang; Hong Wang; Chenghui Huang
• Keywords: TLR9

Lnc-DC is a specific group of long non-coding (Lnc) RNAs in dendritic cells (DCs). Its function has been previously studied, and includes roles cell differentiation the progression some diseases. In this study, we observed critical role regulating differentiation, growth, apoptosis cells.We first isolated peripheral blood mononuclear to culture induce into DCs, which were then co-cultured with hepatitis B virus (HBV)-secreting HepG2.2.15 for detection changes Lnc-DC. The expression levels TLR9, p-STAT3, SOCS3 tested qPCR western blot. MTT assays used analyze proliferation, cycle, apoptosis. We ELISA test TNF-α, IL-1β, IL-6, IL-12p40, IFN-γ.Co-culture HBV-secreting increased level activated TLR9/STAT3 signaling. HBV DNA (IU/ml) was positively correlated further demonstrating that associated immune response HBV. shown regulate signaling cells. More interestingly, regulation controlled by reducing concentration secreted IL-12, IFN-γ, as well increasing IL-1β cells.Lnc-DC important apoptosis, mediated signaling, also This study provides unidentified mechanism underlying