Genetic analysis for the grain number heterosis of a super-hybrid rice WFYT025 combination using RNA-Seq

• DOI: 10.1186/s12284-018-0229-y
• Publication Date: 2018-06-15T02:21:38Z
• Authors: Liang Chen; Jianmin Bian; Shilai Shi; Jianfeng Yu; Hira Khanzada; Ghulam Mustafa Wa...; Changlan Zhu; Xin Luo; Shan Tong; Xiaorong Yang; Xiaosong Peng; Shuang Yong; Qiuying Yu; Xiaopeng He; Junru Fu; Xiaorong Chen; Lifang Hu; Linjuan Ouyang; Haohua He
• Keywords: RNA-Seq

Despite the great contributions of utilizing heterosis to crop productivity worldwide, molecular mechanism remains largely unexplored. Thus, present research is focused on grain number a widely used late-cropping indica super hybrid rice combination in China using high-throughput next-generation RNA-seq strategy. Here, we obtained 872 million clean reads, and at least one read could maps 27,917 transcripts out 35,679 annotations. Transcript differential expression analysis revealed total 5910 differentially expressed genes (DGHP) between super-hybrid Wufengyou T025 (WFYT025) its parents were identified young panicles. Out DGHP, 63.1% had genetic action mode over-dominance, 17.3% complete-dominance action, 15.6% partial-dominance 4.0% an additive action. DGHP significantly enriched carotenoid biosynthesis, diterpenoid biosynthesis plant hormone signal transduction pathways, with key involved three pathways being up-regulated hybrid. By comparing KEGG pathway QTLs associated number, several located same chromosomal segment some these QTLs. Through panicle development transcriptome analysis, conclude that over-dominant effect probably major contributor WFYT025. The sharing location be considered candidate gene provide valuable targets for cloning functional