Genetic diversity of United States Rambouillet, Katahdin and Dorper sheep

• DOI: 10.1186/s12711-024-00905-7
• Publication Date: 2024-07-30T14:03:48Z
• Authors: Gabrielle M. Becker; Jacob W. Thorne; Joan M. Burke; Ronald M. Lewis; David R. Notter; James L. M. Morgan; Christopher S. Sc...; Whit C. Stewart; R. R. Redden; Brenda M. Murdoch
• Keywords: 0301 basic medicine; 570; Sheep; Homozygote; Genetic Variation; Genetics and Genomics; QH426-470; Breeding; SF1-1100; Polymorphism, Single Nucleotide; 630; United States; Animal culture; 03 medical and health sciences; Animal Sciences; Genetics; Animals; Inbreeding; Meat Science; Selection, Genetic; Sheep, Domestic; Research Article

Abstract Background Managing genetic diversity is critically important for maintaining species fitness. Excessive homozygosity caused by the loss of genetic diversity can have detrimental effects on the reproduction and production performance of a breed. Analysis of genetic diversity can facilitate the identification of signatures of selection which may contribute to the specific characteristics regarding the health, production and physical appearance of a breed or population. In this study, breeds with well-characterized traits such as fine wool production (Rambouillet, N = 745), parasite resistance (Katahdin, N = 581) and environmental hardiness (Dorper, N = 265) were evaluated for inbreeding, effective population size (Ne), runs of homozygosity (ROH) and Wright’s fixation index (FST) outlier approach to identify differential signatures of selection at 36,113 autosomal single nucleotide polymorphisms (SNPs).

Results Katahdin sheep had the largest current Ne at the most recent generation estimated with both the GONe and NeEstimator software. The most highly conserved ROH Island was identified in Rambouillet with a signature of selection on chromosome 6 containing 202 SNPs called in an ROH in 50 to 94% of the individuals. This region contained the DCAF16, LCORL and NCAPG genes that have been previously reported to be under selection and have biological roles related to milk production and growth traits. The outlier regions identified through the FST comparisons of Katahdin with Rambouillet and Dorper contained genes with known roles in milk production and mastitis resistance or susceptibility, and the FST comparisons of Rambouillet with Katahdin and Dorper identified genes related to wool growth, suggesting these traits have been under natural or artificial selection pressure in these populations. Genes involved in the cytokine-cytokine receptor interaction pathways were identified in all FST breed comparisons, which indicates the presence of allelic diversity between these breeds in genomic regions controlling cytokine signaling mechanisms.

Conclusions In this paper, we describe signatures of selection within diverse and economically important U.S. sheep breeds. The genes contained within these signatures are proposed for further study to understand their relevance to biological traits and improve understanding of breed diversity.