Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms

• DOI: 10.1186/s12861-019-0183-y
• Publication Date: 2019-03-07T02:02:11Z
• Authors: Livia Goto-Silva; Nadia M. E. Ayad; Iasmin L. Herzog; Nilton P. Silva; Bernard Lamien; Helcio R. B. Orlande; Annie da Costa Souza; Sidarta Ribeiro; Michele Martins; Gilberto B. Domont; Magno Junqueira; Fernanda Tovar-Moll; Stevens K. Rehen
• Keywords: Organoid; Multiplex; Developmental Biology

Organoid cultivation in suspension culture requires agitation at low shear stress to allow for nutrient diffusion, which preserves tissue structure. Multiplex systems organoid have been proposed, but whether they meet similar parameters as the regularly used spinner flask and its correlation with successful generation of brain organoids has not determined.Here we computational fluid dynamics (CFD) simulate two multiplex conditions: steering plates on an orbital shaker use a previously described bioreactor. The bioreactor had speed high regions that may affect cell aggregate growth, depending volume, whereas computed variables were closer those spinning flask.Our protocol improves initial steps standard formation, produced displayed regionalized structures, including retinal pigmented cells. Overall, conclude is cost-effective practical alternative platforms research testing.