Evolution of blue-flowered species of genus Linum based on high-throughput sequencing of ribosomal RNA genes

• DOI: 10.1186/s12862-017-1105-x
• Publication Date: 2017-12-28T10:47:04Z
• Authors: Nadezhda L. Bolsheva; Nataliya V. Melni...; Ilya V. Kirov; Anna S. Speranskaya; Anastasia A. Krin...; Alexey A. Dmitriev; Maxim S. Belenikin; George S. Krasnov; Valentina A. Laku...; Anastasiya V. Sne...; Tatiana A. Rozhmina; Tatiana E. Samatadze; Olga Yu. Yurkevich; Svyatoslav A. Zos...; Аlexandra V. Amosova; Anna V. Kudryavtseva; Olga V. Muravenko
• Keywords: Linum; Molecular Phylogenetics; Concerted evolution

The species relationships within the genus Linum have already been studied several times by means of different molecular and phylogenetic approaches. Nevertheless, a number ambiguities in phylogeny still remain unresolved. In particular, sections Stellerolinum Dasylinum need further clarification. Also, question independence section Adenolinum remains unanswered. Moreover, L. narbonense other require Additionally, origin tetraploid (2n = 30) including cultivated usitatissimum has not explored. present study examines blue-flowered comparisons 5S rRNA gene sequences as well ITS1 ITS2 35S genes. High-throughput sequencing used for analysis multicopy families. addition to analysis, chromosomal localization rDNA sites determined FISH. Our findings confirm that stelleroides forms basal branch from clade flaxes which is independent formed sect. Dasylinum. current approaches, cytogenetic genomic DNA fingerprinting methods applied previously did discriminate certain Adenolinum. allotetraploid its wild ancestor angustifolium could originate either result hybridization two diploid 16) related modern gandiflorum decumbens, or 14). families allowed us make adjustments flax also reveal intra- interspecific divergence sequences.