Transcriptome changes in rice (Oryza sativa L.) in response to high night temperature stress at the early milky stage

• DOI: 10.1186/s12864-015-1222-0
• Publication Date: 2015-01-23T18:25:38Z
• Authors: Jiang-Lin Liao; Hui-Wen Zhou; Qi Peng; Ping-An Zhong; Hong-Yu Zhang; Chao He; Ying-Jin Huang
• Keywords: Illumina dye sequencing; Fold change; Oryza; Grain Quality

Rice yield and quality are adversely affected by high temperatures, especially at night; nighttime temperatures more harmful to grain weight than daytime temperatures. Unfortunately, global consistently increasing an alarming rate the minimum temperature has increased three times as much corresponding maximum over past few decades. We analyzed transcriptome profiles for rice from heat-tolerant -sensitive lines in response night early milky stage using Illumina Sequencing method. The analysis results sequencing data indicated that 35 transcripts showed different expressions between rice, RT-qPCR analyses confirmed expression patterns of selected transcripts. Functional differentially expressed 21 genes have functional annotation their functions mainly involved oxidation-reduction (6 genes), metabolic (7 transport (4 transcript regulation (2 defense (1 gene) photosynthetic processes. Based on genes, possible process regulates these responding stress was further analyzed. This disrupts electron mitochondria, which leads changes concentration hydrogen ions mitochondrial cellular matrix influences activity enzymes TCA its secondary metabolism plant cells. Using technology, differences transcriptomes described here first time. candidate may provide genetic resources be useful improvement characters rice. model proposed is based transcription two lines. In addition, support future studies molecular mechanisms underlying responses