Molecular cloning and expression analysis of WRKY transcription factor genes in Salvia miltiorrhiza

• DOI: 10.1186/s12864-015-1411-x
• Publication Date: 2015-03-16T16:44:20Z
• Authors: Caili Li; Dongqiao Li; Fenjuan Shao; Shanfa Lu
• Keywords: WRKY protein domain; Salvia miltiorrhiza; Cloning (programming)

WRKY proteins comprise a large family of transcription factors and play important regulatory roles in plant development defense response. The gene Salvia miltiorrhiza has not been characterized.A total 61 SmWRKYs were cloned from S. miltiorrhiza. Multiple sequence alignment showed that could be classified into 3 groups 8 subgroups. Sequence features, the domain other motifs are largely conserved with Arabidopsis AtWRKYs. Each group domains contains characteristic sequences, group-specific might attribute to functional divergence WRKYs. A 17 pairs orthologous SmWRKY AtWRKY genes 21 paralogous identified. Maximum likelihood analysis had undergone strong selective pressure for adaptive evolution. Functional suggested subgroup many divergent functions. Various critical amino acids contributed among subgroups detected. Of SmWRKYs, 22, 13, 4 1 predominantly expressed roots, stems, leaves, flowers, respectively. mainly at least two tissues analyzed. In roots treated MeJA, significant changes expression observed 49 which 26 up-regulated, 18 down-regulated, while 5 either up-regulated or down-regulated different time-points treatment. Analysis published RNA-seq data 42 identified yeast extract Ag(+)-responsive. Through systematic analysis, potentially involved tanshinone biosynthesis predicted.These results provide insights conservation diversification useful information further elucidating