Transcriptomic analysis of Litchi chinensis pericarp during maturation with a focus on chlorophyll degradation and flavonoid biosynthesis

• DOI: 10.1186/s12864-015-1433-4
• Publication Date: 2015-03-20T04:06:17Z
• Authors: Biao Lai; Bing Hu; Yong-Hua Qin; Jie-Tang Zhao; Hui-Cong Wang; Gui-Bing Hu
• Keywords: Flavonoid Biosynthesis; RNA-Seq

The fruit of litchi (Litchi chinensis) comprises a white translucent edible aril surrounded by pericarp. pericarp has been the focus studies associated with size, coloration, cracking and shelf life. However, research at molecular level limited lack genomic transcriptomic information. In this study, an analysis transcriptome was performed to obtain information regarding mechanisms underlying physiological changes in pericarp, including those leading surface coloration.Coincident rapid break down chlorophyll, but substantial increase anthocyanins as developed, two major changes, degreening pigmentation were visually apparent. cDNA library three different coloration stages constructed. A total 4.7 Gb raw RNA-Seq data generated then de novo assembled into 51,089 unigenes mean length 737 bp. Approximately 70% (34,705) could be annotated based on public protein databases and, these, 3,649 genes significantly differentially expressed between any stages, while 156 among all stages. Genes encoding enzymes involved chlorophyll degradation flavonoid biosynthesis identified dataset. transcript expression patterns Stay Green (SGR) suggested key role conclusion supported result assay over-expressing LcSGR tobacco leaves. We also found that levels most especially late anthocyanin co-ordinated up-regulated coincident accumulation anthocyanins, candidate MYB transcription factors likely regulate identified.This study provides large collection transcripts profiles maturation processes, coloration. Since annotated, they provide platform for functional within species.