Analysis of whole genome sequencing for the Escherichia coli O157:H7 typing phages

• DOI: 10.1186/s12864-015-1470-z
• Publication Date: 2015-04-07T08:31:11Z
• Authors: Lauren A Cowley; Stephen J Beckett; Margo Chase-Topping; Neil Perry; Tim J Dallman; David L Gally; Claire Jenkins
• Keywords: Phage typing; Multilocus sequence typing

Shiga toxin producing Escherichia coli O157 can cause severe bloody diarrhea and haemolytic uraemic syndrome. Phage typing of E. facilitates public health surveillance outbreak investigations, certain phage types are more likely to occupy specific niches associated with age groups disease severity. The aim this study was analyse the genome sequences 16 (fourteen T4 two T7) phages determine genes responsible for subtle differences in type profiles. were sequenced using paired-end Illumina sequencing at Genome Analysis Centre Animal Health Veterinary Laboratories Agency bioinformatics programs including Velvet, Brig Easyfig used them. A two-way Euclidian cluster analysis highlighted associations between phages. showed that T7 (9 10) differed by only three formed distinct similar genomic sequences: Group 1 (1, 8, 11, 12 15, 16), 2 (3, 6, 7 13) 3 (2, 4, 5 14). scheme exhibited a significantly modular network linked genetic similarity each group showing these specialised infect subset types. Sequencing has enabled us identify variable within how corresponds changes type.