Bimodal dynamics of primary metabolism-related responses in tolerant potato-Potato virus Y interaction

• DOI: 10.1186/s12864-015-1925-2
• Publication Date: 2015-09-19T08:32:18Z
• Authors: Tjaša Stare; Živa Ramšak; Andrej Blejec; Katja Stare; Neža Turnšek; Wolfram Weckwerth; Stefanie Wienkoop; Dominik Vodnik; Kristina Gruden
• Keywords: Potato virus Y; Secondary metabolism

Potato virus Y (PVY) is a major pathogen that causes substantial economic losses in worldwide potato production. Different cultivars differ resistance to PVY, from severe susceptibility, through tolerance, complete resistance. The aim of this study was better define the mechanisms underlying tolerant responses infection by particularly aggressive PVYNTN strain. We focused on dynamics primary metabolism-related processes during infection. A comprehensive analysis dynamic changes metabolism performed, which included whole transcriptome analysis, nontargeted proteomics, and photosynthetic activity measurements cv. Désirée its transgenic counterpart depleted for accumulation salicylic acid (NahG-Désirée). Faster multiplication occurred NahG-Désirée, with these plants developing strong disease symptoms. show while at transcriptional level are extensive bimodal, only partially translated protein level, final functional outcome. Photosynthesis-related genes transiently induced before viral detected it down-regulated later on. This reflected as deficiency apparatus onset only. Interestingly, specific constant up-regulation some RuBisCO transcripts plants, might be important, proteins have been shown interact proteins. In SA-deficient more sensitive NahG-Désirée consistent down-regulation photosynthesis-related detected. reduction photochemical efficiency identified; nontransgenic decrease transient. transient net rate both genotypes same timing, coincided stomatal conductivity. Down-regulation gene expression decreased line other studies reported effects biotic stress photosynthesis. Here, we additionally induction light-reaction components early stages interaction. As already proteins, their overproduction contribute absence symptoms Désirée.