Comparative miRNAs analysis of Two contrasting broccoli inbred lines with divergent head-forming capacity under temperature stress

• DOI: 10.1186/s12864-015-2201-1
• Publication Date: 2015-12-01T11:56:59Z
• Authors: Chi-Chien Chen; Shih-Feng Fu; Monma Norikazu; Yau-Wen Yang; Yu-Ju Liu; Kazuho Ikeo; Takashi Gojobori; Hao-Jen Huang
• Keywords: Inbred strain

MicroRNAs (miRNAs) play a vital role in growth, development, and stress response at the post-transcriptional level. Broccoli (Brassica oleracea L. var italic) is an important vegetable crop, yield quality of broccoli are decreased by heat stress. The inbred lines that capable producing head high temperature summer unique varieties Taiwan. However, knowledge miRNAomes during formation under limited. In this study, molecular characterization two nearly isogenic with contrasting head-forming capacity was investigated. Head-forming better for heat-tolerant (HT) than heat-sensitive (HS) By deep sequencing computational analysis, 20 known miRNAs showed significant differential expression between HT HS genotypes. According to criteria annotation new miRNAs, 24 novel miRNA sequences genotypes were identified. To gain insight into functional significance, 213 potential targets these 44 differentially expressed predicted. These implicated shoot apical phase change, stimulus, hormone energy metabolism. line related autonomous regulation Bo-FT genes less level shock protein as compared HS. For genotypic comparison, set their had consistent patterns various This large-scale unravel regulatory roles underlying capacity.