Genome-wide identification and functional prediction of novel and fungi-responsive lincRNAs in Triticum aestivum

• DOI: 10.1186/s12864-016-2570-0
• Publication Date: 2016-03-18T15:02:36Z
• Authors: Hong Zhang; Weiguo Hu; Jilei Hao; Shikai Lv; Changyou Wang; Wei Tong; Yajuan Wang; Yanzhen Wang; Xinlun Liu; Wanquan Ji
• Keywords: Blumeria graminis; Intergenic region

Stripe rust (Puccinia striiformis f. sp. tritici; Pst) and powdery mildew (Blumeria graminis Bgt) are important diseases of wheat (Triticum aestivum) worldwide. Increasingly evidences suggest that long intergenic ncRNAs (lincRNAs) developmentally regulated play roles in development stress responses plants. However, identification lincRNAs is still limited comparing with functional gene expression. The transcriptome the hexaploid line N9134 inoculated Chinese Pst race CYR31 Bgt E09 at 1, 2, 3 days post-inoculation was recapitulated to detect lincRNAs. Here, 283 differential expressed were identified from 58218 putative lincRNAs, which account for 31.2 % transcriptome. Of which, 254 DE-LincRNAs responded stress, 52 Pst. Among them, 1328 SnRNP motifs (sm sites) detected showed RRU4–11RR sm site element consensus RRU1–9VU1–7RR motifs, where total number uridine more than but less 11. Additionally, 101 DE-lincRNAs predicted as targets miRNA by psRNATarget, while 5 target mimics using mimicry search TAPIR. Taken together, our findings indicate lincRNA played splicesome inter-regulating miRNA. a complex construction mammal model plant. mass sequence data generated this study provide cue future molecular research on wheat–fungus interactions.