Genetics and fine mapping of a purple leaf gene, BoPr, in ornamental kale (Brassica oleracea L. var. acephala)

• DOI: 10.1186/s12864-017-3613-x
• Publication Date: 2017-03-14T03:21:09Z
• Authors: Xiao-ping Liu; Bao-zhen Gao; Feng-qing Han; Zhi-yuan Fang; Li-mei Yang; Mu Zhuang; Hong-hao Lv; Yu-mei Liu; Zhan-sheng Li; Cheng-cheng Cai; Hai-long Yu; Zhi-yuan Li; Yang-yong Zhang
• Keywords: Ornamental plant

Due to its variegated and colorful leaves, ornamental kale (Brassica oleracea L. var. acephala) has become a popular plant. In this study, we report the fine mapping analysis of candidate purple leaf gene using backcross population an F2 derived from two parental lines: W1827 (with white leaves) P1835 leaves).Genetic indicated that trait is controlled by single dominant gene, which named BoPr. Using markers developed based on reference genome '02-12', BoPr was preliminarily mapped 280-kb interval chromosome C09, with flanking M17 BoID4714 at genetic distances 4.3 cM 1.5 cM, respectively. The recombination rate within almost 12 times higher than usual level, could be caused assembly error for '02-12' interval. Primers were designed 'TO1000', another B. genome. Among newly InDel markers, BRID485 BRID490 found closest BoPr, 0.1 0.2 respectively; between 44.8 kb (reference 'TO1000'). Seven annotated genes are located genomic region, only Bo9g058630 shows high homology AT5G42800 (dihydroflavonol reductase), identified as Blast revealed unanchored scaffold (Scaffold000035_P2) confirming existence '02-12'.This study lays foundation cloning functional gene.