Role of the type VI secretion systems during disease interactions of Erwinia amylovora with its plant host

• DOI: 10.1186/s12864-017-4010-1
• Publication Date: 2017-08-17T11:50:17Z
• Authors: Tim Kamber; Joël F. Pothier; Cosima Pelludat; Fabio Rezzonico; Brion Duffy; Theo H. M. Smits
• Keywords: Fire blight; Transcription, Genetic; RNA-sequencing; QH426-470; 03 medical and health sciences; Genetics; Erwinia amylovora; Symbiosis; 2. Zero hunger; 0303 health sciences; Chemotaxis; Motility; Genomics; Plants; Type VI Secretion Systems; 570: Biologie; Phenotype; Flagella; Multigene Family; Host-Pathogen Interactions; TP248.13-248.65; Gene Deletion; Biotechnology; Research Article

Type VI secretion systems (T6SS) are widespread among Gram-negative bacteria and have a potential role as essential virulence factors or to maintain symbiotic interactions. Three T6SS gene clusters were identified in the genome of E. amylovora CFBP 1430, which T6SS-1 T6SS-3 represent complete machineries, while T6SS-2 is reduced its content.To assess contribution T6SSs transcriptomic changes single double mutants two structural genes generated for T6SS-3. Plant assays showed that slightly more virulent apple shoots inducing less disease symptoms on flowers, indicating only minor effect 1430. The mutations led under vitro conditions differential expression type III systems, iron acquisition, chemotaxis, flagellar, fimbrial genes. Comparison planta transcriptome data sets revealed common three processes set chemotaxis motility Additional experiments proved impaired their motility.These results suggest deletion alters metabolic processes. Nevertheless, difference lesion development flower necrosis was indicative influences progression establishment pathogen host plants.