Genome-wide identification of leaf abscission associated microRNAs in sugarcane (Saccharum officinarum L.)

• DOI: 10.1186/s12864-017-4053-3
• Publication Date: 2017-09-25T09:58:47Z
• Authors: Ming Li; Zhaoxu Liang; Shanshan He; Yuan Zeng; Yan Jing; Weikuan Fang; Kaichao Wu; Guanyu Wang; Xia Ning; Lunwang Wang; Song Li; Hongwei Tan; Fang Tan
• Keywords: Saccharum officinarum; MiRBase; KEGG; Abscission; Saccharum; Polyploid

Sugarcane (Saccharum officinarum L.) is an economically important crop, mainly due to the production of sugar and biofuel (Azevedo RA, Carvalho RF, Cia MC, & Gratão PL, Trop Plant Biol 4:42-51, 2011). Grown in tropical subtropical countries, sugarcane a highly polyploid plant with up ten copies each chromosome, which increases difficulties genome assembly genetic, physiologic biochemical analyses. The increasing demands cost harvest require varieties can shed their leaves during maturity time, so it study mechanism leaf abscission sugarcane. To improve understanding miRNA roles abscission, we reported genome-wide characterization miRNAs putative targets using deep sequencing for six small RNA libraries. In total, 93 conserved 454 novel were identified previously transcriptome as reference. Among them, 25 up-regulated 13 down-regulated plants (LASP) compared packaging (LPSP). Target prediction revealed several miRNA-mRNA modules including miR156-SPL, miR319-TPR2, miR396-GRF miR408-LAC3 might be involved abscission. KEGG pathway enrichment analysis showed differentially expressed may regulate pathways like "plant hormone signal transduction" "plant-pathogen interaction", consistent previous study. addition, 96 variant 135 single nucleotide polymorphisms (SNPs). expression confirmed by qRT-PCR. We possible poaceae specific called miR5384 first time not only miR5384, miRNA, but also presented some miR408-LAC These regulation time. All these findings lay ground work future application breeding program benefit research studies miRNAs.