Transcriptomic analysis of adaptive mechanisms in response to sudden salinity drop in the mud crab, Scylla paramamosain

• DOI: 10.1186/s12864-018-4803-x
• Publication Date: 2018-05-31T12:50:59Z
• Authors: Huan Wang; Lei Tang; Hongling Wei; Junkai Lu; Changkao Mu; Chunlin Wang
• Keywords: Scylla paramamosain; Claw

Scylla paramamosain (Crustacea: Decapoda: Portunidae: Syclla De Hann) is a commercially important mud crab distributed along the coast of southern China and other Indo-Pacific countries (Lin Z, Hao M, Zhu D, et al, Comp Biochem Physiol B Mol Biol 208-209:29–37, 2017; Walton ME, Vay LL, Lebata JH, Estuar Coast Shelf Sci 66(3–4):493–500, 2006; Wang Sun B, F, Fish Shellfish Immunol 67:612–9, 2017). While S. euryhaline species, sudden drop in salinity induces negative impact on growth, molting, reproduction, may even cause death. The mechanism osmotic regulation marine crustaceans has been recently under investigation. However, adapting to not reported. In this study, transcriptomics analysis was conducted gills test its adaptive capabilities over 120 h with from 23 ‰ 3 ‰. At level transcription, 135 DEGs (108 up-regulated 27 down-regulated) annotated by NCBI non-redundant (nr) protein database were screened. GO showed that catalytic activity category most participating genes 24 s-tier terms, indicating intracellular metabolic activities enhanced. Of 164 mapped KEGG pathways, seven top 20 pathways closely related Na+ / K+ -ATPase. Seven additional amino acid metabolism-related also found, signaling pathways. Ion transport metabolism key factors regulating adaptation addition several