Global transcriptome analysis and characterization of Dryopteris fragrans (L.) Schott sporangium in different developmental stages

• DOI: 10.1186/s12864-018-4843-2
• Publication Date: 2018-06-18T12:52:41Z
• Authors: Zhen Lu; Qingyang Huang; Tong Zhang; Baozhong Hu; Ying Chang
• Keywords: KEGG; UniGene

Dryopteris fragrans (D. fragrans) is a potential medicinal fern distributed in volcanic magmatic rock areas under tough environmental condition. Sporangia are important organs for reproduction. This study was designed to characterize the transcriptome characteristics of wild D. sporangia three stages (stage A, B, and C) with aim uncovering its molecular mechanism growth development. Using HiSeq 4000, 79.81 Gb clean data (each sample at least 7.95 GB) were obtained from nine samples, being supplied each period, assembled into 94,705 Unigenes, among which 44,006 Unigenes annotated against public protein databases (NR, Swiss-Prot, KEGG, COG, KOG, GO, eggNOG Pfam). Furthermore, we observed 7126 differentially expressed genes (DEG) (Fold Change > 4, FDR < 0.001), 349,885 SNP loci, 10,584 SSRs. DEGs involved DNA replication homologous recombination strongly stage several cutin, suberin wax biosynthesis had undergone dramatic changes during development, consistent morphological observations. responsible secondary metabolism plant hormone signal transduction changed clearly last two stages. those known associated development reproductive flowering plants have also been validated discussed, such as AGL61, AGL62, ONAC010. In particular, Unigene encoding TFL1, an flower–development regulator plants, identified exhibited highest expression level B sporangia. first report on global analysis fragrans. related flower-seed discussed. All observations paraffin slices. The results this provide rare resources further investigation sporangium stress resistance metabolism.