Genome sequence of the entomopathogenic Serratia entomophila isolate 626 and characterisation of the species specific itaconate degradation pathway

• DOI: 10.1186/s12864-022-08938-2
• Publication Date: 2022-10-27T14:04:53Z
• Authors: Amy L. Vaughan; Eric Altermann; Travis R. Glare; Mark R. H. Hurst
• Keywords: 0301 basic medicine; ANZSRC::310509 Genomics; Serratia; ANZSRC::300804 Horticultural crop protection (incl. pests; Entomopathogen; QH426-470; diseases and weeds); Chromosome; 630; 03 medical and health sciences; Genetics; Animals; chromosome; genome; Serratia marcescens; ANZSRC::300409 Crop and pasture protection (incl. pests; 0303 health sciences; Genome; Virulence; ANZSRC::31 Biological sciences; Research; Itaconate; Horizontal gene transfer; entomopathogen; virulence; Coleoptera; itaconate; ANZSRC::310805 Plant pathology; Larva; ANZSRC::32 Biomedical and clinical sciences; horizontal gene transfer; TP248.13-248.65; Biotechnology; Plasmids

Abstract Background Isolates of Serratia entomophila and S . proteamaculans (Yersiniaceae) cause disease specific to the endemic New Zealand pasture pest, Costelytra giveni (Coleoptera: Scarabaeidae). Previous genomic profiling has shown that S. isolates appear have conserved genomes and, where present, plasmids. In absence C. larvae, prevalence reduces in soil over time, suggesting formed a host-specific relationship with To help define potential genetic mechanisms driving retention chronic , genome isolate 626 was sequenced, enabling identification unique chromosomal properties, defining gain/loss accessory virulence factors relevant pathogenicity larvae. Results We report complete sequence 626, causal agent amber The is 5,046,461 bp, 59.1% G + C content encoding 4,695 predicted CDS. Comparative analysis five previously sequenced species, 336X, marcescens Db11, nematodiphila DH-S01, grimesii BXF1, ficaria NBRC 102596, revealed core 1,165 genes shared. Further comparisons between 336X fewer phage-like regions islands less horizontally acquired material. Genomic analyses presence four-gene itaconate operon, sharing similar gene order as Yersinia pestis ripABC complex. Assessment constructed 626::RipC mutant operon confer possible metabolic advantage initial stages infection. Conclusions Evidence presented where, relative encodes phages, alluding limited horizontal transfer Bioassay assessments -mutant targeted mutation degradation region this found reduced capacity replicate post challenge larval host, implicating establishment within host.