Pseudomonas cultivated from Andropogon gerardii rhizosphere show functional potential for promoting plant host growth and drought resilience

• DOI: 10.1186/s12864-022-09019-0
• Publication Date: 2022-11-30T09:06:37Z
• Authors: Soumyadev Sarkar; Abigail Kamke; Kaitlyn Ward; Eli Hartung; Qinghong Ran; Brandi Feehan; Matthew Galliart; Ari Jumpponen; Loretta Johnson; Sonny T.M. Lee
• Keywords: Andropogon; Resilience

Climate change will result in more frequent droughts that can impact soil-inhabiting microbiomes (rhizobiomes) the agriculturally vital North American perennial grasslands. Rhizobiomes have contributed to enhancing drought resilience and stress resistance properties plant hosts. In predicted events of future droughts, how changing rhizobiome under environmental host needs be deciphered. There is also an urgent need identify recover candidate microorganisms along with their functions, involved resilience, enabling successful development synthetic communities.In this study, we used combination cultivation high-resolution genomic sequencing bacterial communities recovered from rhizosphere a tallgrass prairie foundation grass, Andropogon gerardii. We cultivated host-associated microbes artificial drought-induced conditions identified microbe(s) might play significant role gerardii conditions. Phylogenetic analysis non-redundant metagenome-assembled genomes (MAGs) genome interest - MAG-Pseudomonas. Further metabolic pathway pangenome analyses genes pathways related responses including ACC deaminase; nitrogen transformation assimilatory nitrate reductase MAG-Pseudomonas, which associated enhanced tolerance growth for gerardii.Our data indicated MAG-Pseudomonas has functional potential contribute host's during stressful Our study suggested could positive way. The sets construct community To conclude, mediated through deaminase, place microorganism as important aiding stress. This therefore, provided insights into its optimize productivity ever-changing climatic patterns, especially