Genome-wide identification of the Sec14 gene family and the response to salt and drought stress in soybean (Glycine max)

• DOI: 10.1186/s12864-025-11270-0
• Publication Date: 2025-01-25T11:17:12Z
• Authors: Jinyu Zhang; Liying Zou; Li Wang; Dongchao Zhang; Ao Shen; Yongqi Lei; Maoni Chao; Xinjuan Xu; Zhiwei Xue; Zhongwen Huang
• Keywords: Identification; Drought stress

The Sec14 domain is an ancient lipid-binding that evolved from yeast Sec14p and performs complex lipid-mediated regulatory functions in subcellular organelles intracellular traffic. family characterized by a highly conserved domain, ubiquitously expressed all eukaryotic cells has diverse functions. However, the number characteristics of homologous genes soybean, as well their potential roles, remain understudied. In this study, we identified 77 soybean genome were unevenly distributed across 19 chromosomes. Based on classification method used for Arabidopsis members, GmSec14s can be categorized into three classes: GmPITP1 to GmPITP37, GmSFH1 GmSFH25, GmPATL1 GmPATL15. Structural analysis GmSec14 revealed SFH subfamily contained more introns than other subfamilies. A total 10 protein motifs detected within proteins, with each possessing unique motifs. Two tandem duplications 73 segmental among genes. Additionally, large cis-acting elements, particularly those related plant hormones, abundant promoter regions Tissue expression indicated they exhibited distinct tissue-specific patterns. response salt stress, multiple found either upregulated or downregulated. contrast, majority downregulated under drought stress conditions. Notably, 12 significant alterations following suggesting role these mechanisms. Furthermore, interaction network miRNA regulation associated predicted elucidate members. This study provides systematic comprehensive examination gene which will facilitate further functional research roles tolerance.