Lactobacillus acidophilus attenuates Salmonella-induced intestinal inflammation via TGF-β signaling

• DOI: 10.1186/s12866-015-0546-x
• Publication Date: 2015-10-07T09:31:06Z
• Authors: I-Fei Huang; I-Chun Lin; Pei-Feng Liu; Ming-Fang Cheng; Yen-Chen Liu; Yao-Dung Hsieh; Jih-Jung Chen; Chun-Lin Chen; Hsueh-Wei Chang; Chih-Wen Shu
• Keywords: Synbiotics; Prebiotic

Salmonella is a common intestinal pathogen that causes acute and chronic inflammatory response. Probiotics reduce cytokine production serve as beneficial commensal microorganisms in the human gastrointestinal tract. TGF-β (transforming growth factor β)/SMAD NF-κB signaling play important roles inflammation cells. However, involvement of regulating between probiotics not fully understood.L. acidophilus prebiotic inulin were used to treat Caco-2 cells prior infection with Salmonella. The harvested examine cytokines MIR21 expression immunoblotting real-time PCR. SMAD3/4 reporter vectors transfected into monitor TGF-β1 signaling, respectively.In this study, we showed probiotic L. decreased Salmonella-induced activation Expression cytokines, TNF-α IL-8, acidophilus-pretreated was also significantly lower than infected alone. Moreover, elevated pretreated or synbiotic, combination acidophilus, compared untreated S. typhimurium By contrast, SMAD7, target MIR21, accordingly reduced treated synbiotics. Consistent TGF-β1/MIR21 SMAD7 expression, transcriptional activity higher Furthermore, antibody antagonized modulated by cells.Our results suggest pathway may be involved suppressive effects on caused