The ATP-dependent RNA helicase HrpB plays an important role in motility and biofilm formation in Xanthomonas citri subsp. citri

• DOI: 10.1186/s12866-016-0655-1
• Publication Date: 2016-03-22T19:14:58Z
• Authors: Laís Moreira Granato; Simone Cristina P...; Maxuel de Oliveir...; Marco Aurélio Takita; Alessandra Alves ...; Nian Wang; Marcos Antonio Ma...
• Keywords: RNA Helicase A; Transcription

RNA helicases are enzymes that catalyze the separation of double-stranded (dsRNA) using free energy ATP binding and hydrolysis. DEAD/DEAH families participate in many different aspects metabolism, including synthesis, folding, RNA-RNA interactions, localization degradation. Several important bacterial DEAD/DEAH-box have been extensively studied. In this study, we characterize ATP-dependent helicase encoded by hrpB (XAC0293) gene deletion genetic complementation assays. We provide insights into function Xanthomonas citri subsp. investigating roles biofilm formation on abiotic surfaces host leaves, cell motility, virulence citrus canker bacterium growth planta.The is highly conserved sequenced strains Xanthomonas. Mutation (∆hrpB) resulted a significant reduction biofilms leaves. ∆hrpB also exhibited increased dispersion solid medium plates. showed reduced adhesion biotic delayed development disease symptoms when sprayed susceptible Quantitative reverse transcription-PCR assays indicated expression four type IV pili genes. The transcriptional start site fimA (XAC3241) was determined rapid amplification 5'-cDNA Ends (5'RACE). Based results mRNA structure predictions, 5' UTR may contain three loops. HrpB be involved alterations to promote stability RNA.Our data show adherence surfaces. addition, best our knowledge, first time DEAH has implicated regulation