In vivo functional analysis of L-rhamnose metabolic pathway in Aspergillus niger: a tool to identify the potential inducer of RhaR

• DOI: 10.1186/s12866-017-1118-z
• Publication Date: 2017-11-06T05:53:01Z
• Authors: Claire Khosravi; Roland Sándor Kun; Jaap Visser; María Victoria Ag...; Ronald P. de Vries; Evy Battaglia
• Keywords: Rhamnose; Inducer; Gene cluster; Aspergillus niger; Catabolism

The genes of the non-phosphorylative L-rhamnose catabolic pathway have been identified for several yeast species. In Schefferomyces stipitis, all are organized in a cluster, which is conserved Aspergillus niger, except lra-4 ortholog (lraD). A. niger cluster also contains gene encoding responsive transcription factor (RhaR) that has shown to control expression involved release and catabolism.In this paper, we confirmed function first three putative utilisation from through deletion. We explored identity inducer regulator analysis deletion mutants grown transfer experiments L-rhamnonate. Reduced L-rhamnose-induced on lraA lraB strains, but not L-rhamnonate (the product LraB), demonstrate or compound downstream it. these lraC strain show it fact L-rhamnonate.This work showed RhaR beyond dehydratase (LraC) likely be 2-keto-3-L-deoxyrhamnonate.