Temporal dynamics of the plasma microbiome in recipients at early post-liver transplantation: a retrospective study

• DOI: 10.1186/s12866-021-02154-w
• Publication Date: 2021-04-06T19:03:01Z
• Authors: Toshihiko Okumura; Kazuhiro Horiba; Hideya Kamei; Suguru Takeuchi; Takako Suzuki; Yuka Torii; Jun-ichi Kawada; Yoshiyuki Takahashi; Yasuhiro Ogura; Tomoo Ogi; Yoshinori Ito
• Keywords: Immunosuppression

Abstract Background Immunosuppression during liver transplantation (LT) enables the prevention and treatment of organ rejection but poses a risk for severe infectious diseases. Immune modulation antimicrobials affect plasma microbiome. Thus, determining impact immunosuppression on microbiome may be important to understand immunocompetence, elucidate source infection, predict infection in LT recipients. We characterized recipients at early post-LT assessed association between clinical events. Results In this study, 51 patients who received Nagoya University Hospital from 2016 2018 were enrolled. Plasma samples retrospectively collected following time points: 1) within week after LT; 2) 4 ± 1 weeks 3) 8 4) 2 days positive blood culture. A total 111 analyzed using shotgun next-generation sequencing (NGS) with PATHDET pipeline. Relative abundance Anelloviridae , Nocardiaceae Microbacteriaceae Enterobacteriaceae significantly changed postoperative period. Microbiome diversity was higher than that LT. Antimicrobials associated addition, proportion increased lower acute cellular (ACR) non-ACR patients. Sequencing reads bacteria isolated cultures predominantly identified by NGS 16 samples, human herpesvirus 6 detected as causative pathogen one recipient condition. Conclusions The metagenomic technique has great potential revealing is useful comprehensive diagnostic procedure settings. Temporal dynamics specific microorganisms used indirect markers determination immunocompetence ACR