Distribution of serotypes and antibiotic resistance of invasive Pseudomonas aeruginosa in a multi-country collection

• DOI: 10.1186/s12866-021-02427-4
• Publication Date: 2022-01-06T06:02:27Z
• Authors: Shamima Nasrin; Nicolas Hegerle; Shaichi Sen; Joseph Nkeze; Sunil Sen; Jasnehta Permala-...; Myeongjin Choi; James Sinclair; Milagritos D. Tapia; J. Kristie Johnson; Samba O. Sow; Joshua T. Thaden; Vance G. Fowler; Karen A. Krogfelt; Annelie Brauner; Efthymia Protonot...; Eirini Christaki; Yuichiro Shindo; Andrea L. Kwa; Sadia Shakoor; Ashika Singh-Moodley; Olga Perovic; Jan Jacobs; Octavie Lunguya; Raphael Simon; Alan S. Cross; Sharon M. Tennant
• Keywords: Flagellin

Abstract Background Pseudomonas aeruginosa is an opportunistic pathogen that causes a wide range of acute and chronic infections frequently associated with healthcare-associated infections. Because its ability to rapidly acquire resistance antibiotics, P. are difficult treat. Alternative strategies, such as vaccine, needed prevent . We collected total 413 isolates from the blood cerebrospinal fluid patients 10 countries located on 4 continents during 2005–2017 characterized these inform vaccine development efforts. determined diversity distribution O antigen flagellin types antibiotic susceptibility invasive used antibody-based agglutination assay PCR for typing typing. using Kirby-Bauer disk diffusion method. Results Of isolates, 314 (95%) were typed by or ( n = 99). Among 20 serotypes , most common O1, O2, O3, O4, O5, O6, O8, O9, O10 O11; targets would confer protection against more than 80% The type among 386 was FlaB (41%). Resistance aztreonam (56%) common, followed levofloxacin (42%). also found 22% strains non-susceptible meropenem piperacillin-tazobactam. Ninety-nine (27%) our resistant multiple antibiotics. Isolates FlaA2 commonly multidrug p 0.04). Conclusions Vaccines targeting antigens two antigens, FlaA2, offer excellent strategy