Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in China

• DOI: 10.1186/s12866-022-02610-1
• Publication Date: 2022-08-16T09:02:56Z
• Authors: Zun Wu; Yating Wu; Haofeng Gao; Xuexin He; Qiang Yao; Zhanglei Yang; Jinyi Zhou; Linting Ji; Jinwei Gao; Xuying Jia; Yong Dou; Xiaoyu Wang; Peng Shao
• Keywords: Vibrio vulnificus; Epinephelus

Abstract Vibrio vulnificus is a pathogenic bacterium that causes disease in marine fish, affecting fish farming and human health worldwide. In May 2021, the Bohai Bay region, broke out commercially farmed pearl gentian grouper (♀ Epinephelus fuscoguttatus × ♂ lanceolatus ), causing huge economic losses. The diseased had skin lesions, water accumulation their abdomens, showed tissue organ damage. V. biotype 2 has been reported eels other but it less grouper. this study, strain isolated from was identified as EPL 0201 on basis of physiological biochemical characteristics results 16S rRNA gene gyrB sequencing, virulence detection, recursive infection experiments. To gain comprehensive understanding pathogenicity drug resistance strain, whole-genome sequencing performed. Whole-genome analysis map complete. Virulence Factor Database annotation key factor genes vvhA rtxA , which cause host disease. addition, conferring against cephalosporins, aminoglycosides, tetracyclines, sulfonamides. Antimicrobial susceptibility testing confirmed presence these genome. study show multi-drug resistant with high pathogenicity.