Bifidobacterium bifidum reduces oxidative stress and alters gut flora to mitigate acute liver injury caused by N-acetyl-p-aminophenol

• DOI: 10.1186/s12866-025-03775-1
• Publication Date: 2025-02-25T04:32:58Z
• Authors: Juan Yin; Lin Chen; Yiyou Lin; Jiannan Qiu; Fucai Liu; Yuhao Wang; Xiaobing Dou
• Keywords: Bifidobacterium bifidum; Flora; Parasitology

Pharmacologically-induced liver injury from N-acetyl-p-aminophenol (APAP) overdose has become a leading cause of acute failure. Extensive research elucidated the relationship between intestinal microbiota and pathophysiology diseases. The growing body evidence supporting beneficial effects probiotics, coupled with their established safety profile, led to widespread adoption in clinical practice. Among these, Bifidobacterium bifidum garnered substantial attention due its potential hepatoprotective properties, particularly APAP-induced (AILI). However, precise therapeutic underlying mechanisms alleviate drug-induced toxicity remain largely unexplored. To address this knowledge gap, present study aimed investigate role new strain CGMCC No. 29,545 isolated faeces on AILI. A mouse model was constructed through administration heat-killed or active B. preparations via gavage, followed by an intraperitoneal APAP. results showed that could significantly reverse increase plasma transaminase levels reduce necrotic area cells AILI mice. reduction oxidative stress accompanied effect. Furthermore, attenuated endotoxin improved colonic inflammation, reducing hepatocyte apoptosis. 16 S rRNA diversity contents suggests involvement regulation also plays crucial protection against above suggest amelioration multiple injuries APAP overprocessing is closely related bifidum, which confirmed preparations. Heat-killed did not attenuate degree caused treatment. two different provide insights into protective strategies as probiotic